Part:BBa_K136057:Design

RBS - lasI - GFP tTripart

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 679
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 241
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1358
  

Design Notes

gfp tripart (backward insert) was cloned into pSB1A2 containing RBS-lasI

Source

Assembling of parts from the registry